HLA-DRB1 Antibody (MHC II)

SKU: orb749570

描述

This mAb reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Its epitope is different from that of mAb L243. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.

研究领域

Cell Biology

图片与验证

−

Item 1 of 6

已测应用	FACS, IF, IHC-P, WB
稀释范围	Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
反应性	Human
应用说明	Optimal dilution of the HLA-DRB1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

−

抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG2b, kappa
克隆号	HLA-DRB/1067
免疫原	Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DRB1 antibody.
纯化	Protein G affinity chromatography
偶联	Unconjugated

储存与操作

−

储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
有效期	12 months from date of receipt.
免责声明	For research use only

相似产品

−

Item 1 of 10
HLA-DPB1 Antibody [orb608003]
应用：
ELISA, FC, IF, IHC, WB
反应性：
Human
种属/宿主：
Rabbit
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 20 μg, 100 μg, 100 μg (without BSA and Azide)
Item 1 of 8
HLA-DPB1 Antibody [orb607962]
应用：
FC, IF, IHC, WB
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 20 μg, 100 μg, 100 μg (without BSA and Azide)
Item 1 of 6
HLA-DRA Antibody [orb2312526]
应用：
IHC, WB
反应性：
Human
种属/宿主：
Rabbit
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 20 μg, 100 μg, 100 μg (without BSA and Azide)
Item 1 of 6
HLA-DRB1 Antibody [orb2312931]
应用：
IHC, WB
反应性：
Human
种属/宿主：
Rabbit
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 20 μg, 100 μg, 100 μg (without BSA and Azide)
Item 1 of 4
HLA-DRA Antibody [orb1410572]
应用：
FC, IF, IHC, WB
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 20 μg, 100 μg, 100 μg (without BSA and Azide)

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.