Human High Affinity IgE Receptor Alpha Chain (FceRIa) Recombinant Protein (Biotin)

SKU: orb3245190

描述

Recombinant fusion protein consisting of two copies of the extracellular part of human high affinity IgE receptor α-chain fused to human serum albumin (HSA) [FcεRIα-HSA-FcεRIα]. The protein is secreted from a recombinant CHO cell line and specifically binds to human immunoglobulin E (IgE) with affinity comparable to the native IgE receptor expressed on mast cells and basophils (KD < 1nM). Among other applications it can be used as functional reagent in allergy research, mast cell / basophil biology and as analytical tool for detection and quantification of biologically active (= receptor binding) IgE in experimental cell supernatants and body fluids.

图片与验证

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已测应用	ELISA, FA, FACS, WB
应用说明	This product is for research use only. Follow Standard Laboratory Practice when handling this recombinant protein. The product contains 0.1 % sodium azide (NaN3) as a preservative, which has been classified as Xn (harmful) in the European Directive 67/548/EEC and should be handled with care. Flush intensively with water when disposing of this reagent through copper or lead plumbing.

关键属性

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来源	Recombinant protein produced in CHO cells
纯化	Affinity - purified from cell culture supernatant by Immunoaffinity. Chromatography on CH-Sepharose 4B – mouse anti-human FcεRIα mAb (IM-2). Biotinylated with Long Chain Biotin.
纯度	> 90%, tested by analytical Gel Permeation Chromatography

储存与操作

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储存	Store undiluted at 4°C. For long term storage, aliquot and store at -20°C or -80°C. Avoid repeated freeze-thawing. The product may be shipped on ice pack or dry ice.
缓冲液/防腐剂	Phosphate buffered saline (PBS); pH = 7.2; 0.1% NaN3 (sodium azide)
浓度	0.5 mg/ml (Bradford)
有效期	6 months from date of receipt.
免责声明	For research use only

相似产品

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Human High Affinity IgE Receptor Alpha Chain (FceRIa) Recombinant Protein (Biotin) [orb3245192]
偶联：
Biotin
可选规格： 50 μg, 100 μg

质量保证

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