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Human IgG Antibody Phycoerythrin conjugated Pre-Adsorbed

SKU: orb347200

描述

HUMAN IgG (H&L) (GOAT) antibody (RPE)

图片与验证

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已测应用DOT, IF
稀释范围IF: 1:100 - 1:250
反应性Human
应用说明
Anti-Human IgG (H&L) Phycoerythrin has been tested by dot blot and is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

关键属性

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抗体类型Secondary Antibody
宿主Goat
克隆性Polyclonal
同种型IgG
免疫原Anti-Human IgG whole molecule was produced by repeated immunization with Human IgG whole molecule in goat.
纯度This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
偶联RPE

储存与操作

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储存Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
形态/外观Lyophilized
缓冲液/防腐剂Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度0.5 mg/mL
有效期12 months from date of receipt.
免责声明For research use only

别名

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goat anti-Human IgG phycoerythrin conjugated Antibody, goat anti-Human IgG Antibody PE conjugation

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质量保证

质量保证

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实验方案信息

IF
免疫荧光
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DOT
斑点印迹(Dot Blot)
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可选规格

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1 ml
¥ 7,800.00
免费二抗(20 µl)0/0

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