Recombinant Cytokeratin 8 Antibody / Rabbit Monoclonal

SKU: orb2640541

描述

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibody that recognizes only cytokeratin 8 and 18. Cytokeratin 8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium.

图片与验证

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已测应用	IHC-P, WB
稀释范围	Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Western blot: 1-2ug/ml
反应性	Human
应用说明	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Cytokeratin 8 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型	Primary Antibody
宿主	Rabbit
克隆性	Recombinant
同种型	Rabbit IgG, kappa
克隆号	KRT8/2174R
免疫原	A cytoskeletal preparation from human HeLa cells was used as the immunogen for this recombinant Cytokeratin 8 antibody.
纯化	Protein A affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
有效期	12 months from date of receipt.
免责声明	For research use only

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IHC-P, WB
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Recombinant Cytokeratin 8 Antibody / Rabbit Monoclonal [orb2640540]
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IHC-P
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Recombinant Cytokeratin 8 Antibody / Rabbit Monoclonal [orb2643688]
应用：
IHC-P
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质量保证

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