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Luciferase Antibody

SKU: orb334957

描述

Goat polyclonal antibody to Firefly luciferase. Luciferase is a widely used reporter protein to study gene expression. Luciferase catalyses a bioluminescent reaction which requires luciferin as a substrate, ATP and Mg2+. A cell extract containing luciferase mixed luciferin in the presence of ATP and Mg2+, results in a flash of light that decays rapidly and can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

图片与验证

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已测应用WB
稀释范围WB:1:500-1:2,000
反应性Insect
应用说明
The antibody solution should be gently mixed before use.

关键属性

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宿主Goat
克隆性Polyclonal
同种型IgG
免疫原Antigen: Purified recombinant peptide derived from within residues 340 aa to the C-terminus of Luciferase produced in E. coli. Antigen Sequence: LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL
靶点Firefly Luciferase
纯化Epitope affinity purified
偶联Unconjugated

储存与操作

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储存Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
形态/外观Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
浓度1 mg/ml
有效期12 months from date of receipt.
免责声明For research use only

别名

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Luc and Luciferin 4-monooxygenase antibody.

相似产品

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质量保证

质量保证

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实验方案信息

WB
免疫印迹(WB)
查看实验方案

可选规格

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100 μg
¥ 3,640.00
选择偶联或无载体版本
免费二抗(20 µl)0/0

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发货Usually dispatched within 2-3 days
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