LY-294002 hydrochloride

SKU: orb1303185

描述

LY-294002 hydrochloride is a potent and cell-permeable inhibitor of phosphoinositide 3-kinase (PI3K) isoforms α, δ, and β. It is widely used in vitro to study PI3K signaling pathways in cancer and cellular metabolism, and it also inhibits autophagosome formation, facilitating autophagy research.

研究领域

Cell Biology, Metabolism Research, Molecular Biology, Signal Transduction, Stem Cell & Developmental Biology

图片与验证

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关键属性

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CAS 号	934389-88-5
MW	343.81
纯度	99.98% (May vary between batches)
分子式	C₁₉H₁₇NO₃·HCl
SMILES	Cl.O=c1cc(oc2c(cccc12)-c1ccccc1)N1CCOCC1
靶点	PI3K,Casein Kinase,DNA-PK,Apoptosis
溶解性	DMSO:50 mg/mL (145.43 mM);Ethanol:5 mM

生物活性

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靶点 IC50	PI3Kα:0.5μM\|PI3Kβ:0.97 μM\|CK2α2:3.869 μM\|CK2:0.098 μM\|PI3Kδ:0.57μM
体内	LY294002 can suppress tumor growth and induce apoptosis, especially in the LoVo tumors. Thus, It shows remarkable effectiveness in the mouse peritonitis carcinoma tosa model . LY294002 markedly inhibits growth and ascites formation of ovarian carcinoma .
体外	LY294002 inactivates Akt/PKB, consequently inhibiting cell proliferation and inducing apoptosis. In these colon cancer cell lines, LY294002 shows a remarkable growth-inhibitory and apoptosis-inducing effect and decreases expression of phosphorylated Akt (Ser473). LY294002 induces diminished cytoplasmic volume and obvious nuclear pyknosis in the tumor cells. LY294002 induces specific G1 arrest in cell growth, leading to almost complete inhibition of melanoma cell proliferation and partial inhibition of MG-63 (osteosarcoma cell line) proliferation.
细胞研究	Cell lines: Colon cancer cell lines DLD-1,LoVo,HCT15,and Colo205. Concentrations: 0–50 μM. Incubation Time: 0–48 hours. Method: 1.0×105 cells (100 μL volume/well) are inoculated into 96-well microtiter plates.LY294002 is added to triplicate wells and cultured at 37oC for 0–48 hours.After treatment,Premix WST-1 (10 μL) is added to each microculture well,and the plates are incubated for 60 minutes at 37oC,after which absorbance at 450 nm is measured with a microplate reader.
动物研究	Animal Models: Two groups of athymic nude mice (5–7 weeks) are inoculated i.p.with OVCAR-3 cells. Formulation: Dissolved in DMSO plus 0.25 ml of PBS. Dosages: 0–100 mg/kg. Administration: i.p.

储存与操作

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储存	-20°C
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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spermatozoa, reversibly, phosphatidylinositol 3-kinase, SF1101, SF-1101, p110δ, p110β, p110α, tumor, translocation, CNE-2Z, broad-spectrum, Human, Leptin, LY 294002, LY 294002 hydrochloride, LY-294002, LY294002 hydrochloride, LY-294002 Hydrochloride, LY294002, Lysophosphatidic acid, nasopharyngeal, nuclear, NSC697286, NSC-697286, NSC 697286, NPC

质量保证

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化合物标识

PubChem CID

11957589 ↗

关键属性

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暂无计算属性。

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LY-294002 hydrochloride

描述

研究领域

图片与验证

关键属性

生物活性

储存与操作

别名

质量保证

化合物标识

关键属性

实验方案信息

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