Maltose Binding Protein (MBP) Epitope Tag Antibody Biotin Conjugated

SKU: orb344774

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描述

MBP Epitope Tag antibody (Biotin)

研究领域

Pharmacology & Drug Discovery

图片与验证

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Item 1 of 3

已测应用	ELISA, WB
稀释范围	ELISA: 1:10,000-1:50,000, WB: 1:1,000-1:5,000
应用说明	Anti-MBP Biotin Conjugated Antibody is optimally suited for monitoring the expression of MBP tagged fusion proteins. As such, anti- MBP/MBP can be used to identify fusion proteins containing the MBP epitope. The antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against MBP containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions. Anti-MBP is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli. By Western blot, a band is seen at ~ 42 kDa representing MBP.

关键属性

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抗体类型	Primary Antibody
宿主	Rabbit
克隆性	Polyclonal
同种型	IgG
免疫原	This antibody was purified from whole rabbit serum prepared by repeated immunizations with the MBP epitope tag recombinant protein.
纯度	This IgG purified antibody is directed against MBP and is useful in determining its presence in various assays. This polyclonal anti-MBP tag antibody detects over-expressed proteins containing the MBP epitope tag. To date this antibody has reacted with all MBP tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins.
偶联	Biotin

储存与操作

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储存	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
形态/外观	Lyophilized
缓冲液/防腐剂	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度	1.0 mg/mL
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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rabbit anti-MBP Epitope Tag Antibody biotin conjugated, rabbit anti-Maltose Binding Protein Antibody biotin conjugation

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Item 1 of 3
Maltose Binding Protein (MBP) Epitope Tag Antibody Biotin Conjugated [orb344775]
应用：
ELISA, WB
种属/宿主：
Rabbit
克隆性：
Polyclonal
偶联：
Biotin
可选规格： 25 μl

质量保证

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