Mouse anti Human IgM (class specific) Antibody (Biotin)

SKU: orb22283

描述

Biotin-conjugated purified monoclonal mouse antibody to human IgM, isotype specific. To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgM, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:100 to 1:500; in ELISA from 1:1,000 upwards; in Western blotting from 1:2,000 upwards. These data should be interpreted as general recommendations only. Working dilutions may vary widely, strongly depending on the test conditions.

研究领域

Stem Cells

图片与验证

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反应性	Human

关键属性

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抗体类型	Secondary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	IgG1
克隆号	NI 179
偶联	Biotin

储存与操作

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储存	The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.
形态/外观	Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
有效期	12 months from date of receipt.
免责声明	For research use only

质量保证

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实验方案信息

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