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Mouse Gamma-Aminobutyric Acid (gABA) ELISA Kit

SKU: orb782385
ELISA 试剂盒促销
特色特色

描述

The Mouse Gamma-Aminobutyric Acid (gABA) ELISA Kit is designed to quantify Gamma-Aminobutyric Acid (gABA) in mouse serum, plasma, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 31.25-2000 pg/mL and a sensitivity of 9.66 pg/mL.

研究领域

Neuroscience; Pharmacology & Drug Discovery

图片与验证

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已测应用ELISA
应用说明
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse gABA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse gABA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse gABA in the samples is then determined by comparing the OD of the samples to the standard curve.

关键属性

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反应性Mouse
样本类型Serum, plasma, cell lysates, cell culture supernates and other biological fluids
检测类型Competitive
检测时间2h
范围31.25-2000 pg/mL
灵敏度9.66 pg/mL
浓度2000 pg/mL

步骤与表现

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Assay Principle
This kit is based on a competitive ELISA detection method. The microtiter plate is pre-coated with target antigen. During the assay, the target antigen in the sample or standard competes with the immobilized target for binding sites on the biotinylated detection antibody specific to the target antigen. After incubation, excess conjugate and unbound sample or standard are removed by washing. HRP–streptavidin (SABC) is then added to each well and incubated, followed by the addition of TMB substrate solution. The enzymatic reaction is terminated by adding an acidic stop solution, and the color change is measured at 450 nm using a microplate reader. The concentration of the target antigen in the samples is determined by comparison with the standard curve and is inversely proportional to the OD450 value.
Kit Components
1. ELISA Microplate
2. Standards
3. Detection Antibody
4. HRP-Streptavidin Conjugate
5. TMB Substrate
6. Dilution buffers
7. Stop Solution
8. Wash Buffer
9. Plate Sealers
10. Manual
Reagent Preparation
1. Wash Buffer: Prepare the 1X Wash Buffer using distilled water according to the manual.
2. Standard: Perform gradient dilution according to the instructions in the manual.
3. Other Concentrated Reagents: Dilute the concentrated reagents using the Dilution Buffers provided in the kit to 1 X working solutions as instructed in the manual. Always use a clean pipette tip for each different solution.
Assay Procedure
This procedure is for reference only.

1. After the kit equilibrates to room temperature, add standards or samples to each well and incubate.
2. Add biotinylated antibody working solution to each well and incubate.
3. Discard liquid, add wash buffer to each well, wash the plate three times, and blot dry on clean absorbent paper.
4. Add streptavidin-HRP working solution to each well and incubate._x000b_5. Discard liquid, add wash buffer to each well, wash the plate five times, and blot dry on clean absorbent paper.
6. Add TMB substrate solution to each well and incubate in the dark.
7. Add stop solution to each well, mix thoroughly, and immediately read OD at 450 nm.
Materials Required
1. Microplate readers
2. Centrifuge
3. Incubator
4. Automated plate washer
5. Single-channel or multi-channel high-precision pipettes
6. Disposable pipette tips
7. Sterile tubes
8. Eppendorf tubes
9. Absorbent paper
10. Loading slots
Precision
Intra-assay Precision (Precision within an assay): CV% < 8%
Intra-assay precision was evaluated by testing multiple replicates of samples within the same plate.

Inter-assay Precision (precision between assays): CV% < 10%
Inter-assay precision was evaluated by testing samples across different plates.
Calculation of Results
1. Average the duplicate readings for each Standard, Control, and Sample, and subtract the mean optical density of the zero Standard.
2. Construct a standard curve by plotting the target concentration on the y-axis against absorbance on the x-axis and draw a curve through the data points.
3. Determine the sample concentration by substituting the OD450 value into the standard curve. For diluted samples, multiply the calculated value by the corresponding dilution factor.
Curve Fitting Softwares
1. Curve Expert
2. Thermo SkanIt RE
3. SciDAVis
4. LabPlot
5. ……

客户验证数据

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以下数据由各地研究人员提交。即使我们尚未自行检测，这些结果也有助于判断该产品是否适合您的应用或种属。
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反应性分布

最新实验

1 Results
稀释
样本
摘要
ELISA
Mouse
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Hippocampal tissue lysates
This kit was used to measure...

储存与操作

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储存请参阅操作手册中的储存说明
有效期Please enquire.
免责声明For research use only

别名

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4-Aminobutyric Acid ELISA Kit

相似产品

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质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

基因符号
gABA

实验方案信息

ELISA
酶联免疫吸附测定（ELISA）
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Jiushuang Zhu 1, Zhuoting Zhong 1, Lijie Shi 1, Ling Huang 1, Chunqiao Lin 1, Yan He 1, Xiuwen Xia 1, Tiane Zhang 1, Weijun Ding 1, Youjun Yang Gut microbiota mediate early life stress-induced social dysfunction and anxiety-like behaviors by impairing amino acid transport at the gut Gut Microbes, (2024)

PubMed: 39259834

应用

ELISA

反应性

Mouse

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