Mouse IgG2b Isotype Control Biotin Antibody

SKU: orb154468

描述

Mouse monoclonal antibody conjugated to Biotin which recognizes IgG2b that belongs to many immunoglobulin G. It can be used as a negative control, to distinguish specific from non-specific binding of mouse IgG2b fluorochrome-conjugated antibodies to human cells. It was found that IgG2a was superior to IgG1 in activating complement. IgG2 is an antibody moleculer induced by infection.

图片与验证

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Item 1 of 1

已测应用

ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB

应用说明

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

关键属性

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抗体类型	Secondary Antibody
克隆性	Monoclonal
同种型	Mouse IgG2b kappa
克隆号	MPC-11
免疫原	KLH-coupled trinitrophenol
纯化	Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
偶联	Biotin

储存与操作

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储存	Store at 2-8°C. Do not freeze.
缓冲液/防腐剂	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
浓度	1 mg/ml
有效期	12 months from date of receipt.
免责声明	For research use only

相似产品

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Mouse IgG2b isotype control Biotin Antibody [orb343790]
应用：
FC, IF
偶联：
Biotin
可选规格： 100 μg

质量保证

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