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Mouse IgG2b Isotype Control PerCP Antibody

SKU: orb154540

描述

Mouse monoclonal antibody conjugated to PerCP which recognizes IgG2b that belongs to many immunoglobulin G. It can be used as a negative control, to distinguish specific from non-specific binding of mouse IgG2b fluorochrome-conjugated antibodies to human cells. It was found that IgG2a was superior to IgG1 in activating complement. IgG2 is an antibody moleculer induced by infection.

图片与验证

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已测应用FC
应用说明
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

关键属性

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抗体类型Secondary Antibody
克隆性Monoclonal
同种型Mouse IgG2b kappa
克隆号MPC-11
免疫原KLH-coupled trinitrophenol
纯化Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
偶联PerCP

储存与操作

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储存Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
缓冲液/防腐剂Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
浓度0.1 mg/ml
有效期12 months from date of receipt.
免责声明For research use only

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NCBI 基因详情

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实验方案信息

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可选规格

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0.1 mg
¥ 4,810.00
免费二抗(20 µl)0/0

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发货Usually dispatched within 5-10 working days weeks.
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