MYC Epitope Tag Antibody Biotin Conjugated

SKU: orb345879

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描述

MYC Epitope Tag antibody (Biotin)

研究领域

Cell Biology

图片与验证

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已测应用	DOT, ELISA, IHC, WB
稀释范围	ELISA: 1:100,000 - 1:500,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
应用说明	Anti-myc has utility to detect the fusion protein of the myc epitope cloned along with the target gene. As such, anti-myc/myc can be used to identify fusion proteins containing the myc epitope. The antibody recognizes the Myc tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and myc containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. Biotin conjugated anti-EPITOPE tag antibodies are generally suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.

关键属性

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抗体类型	Primary Antibody
宿主	Rabbit
克隆性	Polyclonal
同种型	IgG
免疫原	This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
纯度	This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum.
偶联	Biotin

储存与操作

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储存	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
形态/外观	Lyophilized
缓冲液/防腐剂	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度	1.0 mg/mL
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Rabbit anti-MYC Epitope Tag Biotin Conjugated Antibody, Rabbit anti-c-myc tag Biotin conjugation, Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu

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质量保证

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