Necrosis vs Apoptosis Assay Kit

SKU: orb1409904

描述

This kit simultaneously detects both cell death due to both apoptosis and necrosis. It can be used to assess the effects of novel therapeutic agents on cell health. Analyze the fluorescent signal using fluorescence microscopy or flow cytometry.

研究领域

Cell Biology

图片与验证

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应用说明

1. Prepare samples and controls. 2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20. 3. Reconstitute FLICA with 50 μL DMSO. 4. Dilute FLICA 1:5 by adding 200 μL PBS. 5. Add diluted FLICA to each sample at 1:30 – 1:60. For example, to stain at 1:30, add 10 μL to 290 μL of cultured cells. To stain at 1:60, add 5 μL to 295 μL of cultured cells. 6. Incubate approximately 1 hour. 7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells. 8. If desired, label with additional stains, such as Hoechst, 7-AAD, or an antibody. 9. If desired, fix or embed cells. 10. Analyze with a fluorescence microscope, fluorescence platereader, or flow cytometer. SR-FLICA excites at 550-580 nm and emits at 590-600 nm.

关键属性

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样本类型	Cell culture

储存与操作

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储存	2-8°C
有效期	6 months from date of receipt.
免责声明	For research use only

相似产品

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Necrosis vs Apoptosis Assay Kit [orb1409905]
可选规格： 50 - 100 T
Necrosis vs Apoptosis Assay Kit [orb746805]
可选规格： 50 - 100 T, 100 - 200 T

质量保证

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