Neomycin sulfate

SKU: orb1310138

描述

Neomycin sulfate is an aminoglycoside antibiotic that inhibits bacterial protein synthesis. It is widely used as a selection agent in molecular biology to identify cells transfected with neomycin resistance genes, applicable in both in vitro cell culture and in vivo animal model studies.

研究领域

Infectious Disease & Virology, Metabolism Research

图片与验证

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关键属性

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CAS 号	1405-10-3
MW	908.87
纯度	99.88% (May vary between batches)
分子式	C₂₃H₄₆N₆O₁₃·3H₂SO₄
SMILES	OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.NC[C@@H]1O[C@H](O[C@@H]2[C@@H](CO)O[C@@H](O[C@@H]3[C@@H](O)[C@H](N)C[C@H](N)[C@H]3O[C@H]3O[C@H](CN)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O)[C@H](N)[C@@H](O)[C@@H]1O
靶点	Phospholipase,Antibiotic,Antibacterial,ribosome
溶解性	DMSO:Insoluble;PBS:100 mg/mL (110.03 mM);H2O:460 mg/mL (506.12 mM)

生物活性

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靶点 IC50	PC-PLD:65 µM\|ATP-induced current:90 μM
体内	METHODS: To assay in vivo activity, Neomycin sulfate (20 mg/mouse) and bacitracin (20 mg/mouse) were administered by gavage to C57BL/10 mice once daily for seven days. RESULTS: The combination of Neomycin and bacitracin decreased intestinal permeability and increased gene expression of ZO-1, JAM-A, and occludin in the ileum and ZO-1, claudin-3, and claudin-4 in the colon. METHODS: To deplete the intestinal microbiota of mice, the antibiotics (ABX) vancomycin (0.5 g/L), ampicillin (1 g/L), Neomycin sulfate (1 g/L), and metronidazole (1 g/L) were administered to mice by drinking water for two weeks. RESULTS: Antibiotics significantly reduced the diversity and composition of the gut microbiota.
体外	METHODS: BHK-21 cells, VERO cells and FEA cells were treated with Neomycin sulfate (1000-20000 μg/mL) for 24 h. Cell viability was measured by MTT. RESULTS: The viability of BHK-21 cells was significantly reduced by 9000-20000 μg/mL Neomycin treatment. 3000 μg/mL Neomycin treatment significantly reduced the viability of FEA cells. 20000 μg/mL Neomycin treatment did not affect the viability of VERO cells. METHODS: Mouse cochlear hair cells HEI-OC-1 were treated with Neomycin sulfate (2 mM) for 24 h. Apoptosis was detected by Flow Cytometry. RESULTS: 20 μM GM1 significantly reduced Neomycin sulfate-induced cell death in HEI-OC-1 cells.

储存与操作

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储存	-20°C
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Framycin Sulfate, Framycin sulfate, Inhibitor, inhibit, Neomycin, Neomycin sulfate, Neomycin Sulfate, Neomycin sulphate, Antibiotic, angiogenin, aminoglycoside, Bacterial, 30S ribosome, synthesis, ribosomal, proliferation, protein, Phospholipase

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Neomycin sulfate ELISA kit [orb3186096]
反应性：
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动态范围：
39.063-2500pg/ml
灵敏度：
23.438pg/ml
可选规格： 48 T, 96 T
Framycetin sulfate [orb1306492]
纯度：
99.89% (May vary between batches)
CAS 号：
4146-30-9
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C₂₃H₅₂N₆O₂₅S₃
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Neomycin sulfate [orb1226348]
纯度：
>98% (HPLC)
CAS 号：
1405-10-3
MW:
908.87
分子式：
C₂₃H₄₆N₆O₁₃·3H₂SO₄
可选规格： 10 mg, 25 mg, 50 mg, 200 mg, 100 mg, 500 mg, 1 g
G-418 disulfate [orb1227019]
纯度：
>98% (HPLC)
CAS 号：
108321-42-2
MW:
692.7094
分子式：
C₂₀H₄₄N₄O₁₈S₂
可选规格： 1 g, 500 mg

质量保证

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化合物标识

PubChem CID

24978553 ↗

关键属性

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暂无计算属性。

实验方案信息

查看更多实验方案

反应性：	All
动态范围：	39.063-2500pg/ml
灵敏度：	23.438pg/ml

纯度：	99.89% (May vary between batches)
CAS 号：	4146-30-9
MW:	908.86
分子式：	C₂₃H₅₂N₆O₂₅S₃

纯度：	>98% (HPLC)
CAS 号：	1405-10-3
MW:	908.87
分子式：	C₂₃H₄₆N₆O₁₃·3H₂SO₄

Neomycin sulfate

描述

研究领域

图片与验证

关键属性

生物活性

储存与操作

别名

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