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描述
研究领域
Neuroscience
图片与验证
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Item 1 of 6
| 已测应用 | ELISA, IHC, WB |
|---|---|
| 稀释范围 | ELISA: 1:20,000 - 1:80,000, IHC: 5ug/ml, WB: 1:500 - 1:3,000 |
| 反应性 | Human, Mouse |
| 应用说明 |
关键属性
−| 抗体类型 | Primary Antibody |
|---|---|
| 宿主 | Rabbit |
| 克隆性 | Polyclonal |
| 同种型 | IgG |
| 免疫原 | Anti-Nestin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1484-1500 of human Nestin protein. |
| 靶点 | NES |
| 纯度 | This affinity purified antibody is directed against human Nestin protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Nestin protein from human and Mesocricetus auratus (100% homology). Based on protein sequence homology also expect partial reactivity against Nestin homologues from mouse (93%) and rat (88%). Reactivity against homologues from other sources is not known. |
| 偶联 | Unconjugated |
储存与操作
−| 储存 | Store anti-Nestin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| 形态/外观 | Liquid (sterile filtered) |
| 缓冲液/防腐剂 | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| 浓度 | 1.23 mg/mL |
| 有效期 | 12 months from date of receipt. |
| 干冰运输 | 请注意：本产品需要干冰运输，将收取干冰附加费。 |
| 免责声明 | For research use only |
别名
−rabbit anti-Nestin antibody, rabbit anti-NES antibody, Nbla00170
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质量保证
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