Nucleoli Marker Antibody / Human Nucleolar Antigen

SKU: orb248680

描述

This mAb is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone NM95 antibody recognizes an antigenic marker associated with the nucleoli in human cells. The antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells. The nucleoli marker antibody can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

研究领域

Immunology & Inflammation

图片与验证

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Item 1 of 7

已测应用	FACS, ICC, IF, IHC-P
稀释范围	Flow cytometry: 1-2ug/10^6 cells ,Immunofluorescence: 1-2ug/ml,Immunocytochemistry: 1-2ug/ml for 30 min at RT,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
反应性	Human
应用说明	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nucleoli marker antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG1, kappa
克隆号	NM95
免疫原	Nuclei of myeloid leukemia biopsy cells were used as the immunogen.
纯化	Protein G affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
有效期	12 months from date of receipt.
免责声明	For research use only

相似产品

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Item 1 of 7
Nucleoli Marker Antibody / Human Nucleolar Antigen [orb2638091]
应用：
FACS, ICC, IF, IHC-P
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 μg
Item 1 of 4
Nucleoli Marker Antibody / Human Nucleolar Antigen [orb2638090]
应用：
IHC-P
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 7 ml

质量保证

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