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One step Polymer HRP Goat IgG (H+L)

SKU: orb2652844

描述

IBSC One-Step rabbit anti-Goat IgG (H+L); biotin/avidin free system stains membranes, cytoplasmic and nuclear antigens. It provides the user with a rapid and easy to use IHC detection system.

研究领域

Cell Biology

图片与验证

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One step Polymer HRP Goat IgG (H+L)
应用说明
Procedure: IHC/ICC procedure for frozen, paraffin sections and cell smears. 1. Deparafinize and hydrate tissue sections through xylene or other clearing agents and graded alcohols. 2. Wash 2-3 with distilled or deionized water. 3. Incubate sections/cell smear with Endoblocker for 5-10 minutes at room temperature. Wash with distilled water 3X. 4. Note: If antigen retriever is required it can be applied at this step. Please refer to data sheet for the primary antibody. 5. Wash slide with PBS or Tris saline buffer or washing buffer 3X. 6. Incubate sections/ cell smear in Protein blocking solution, for 5-10 minutes at room temperature. 7. Incubate sections/cell smear with primary antibody for 20-30 minutes. 8. Wash slide with PBS 5-7X. 9. Incubate with One-Step HRP polymer for 20-30 minutes at room temperature. 10. Wash slide 5-7 times with buffer. Caution: Peroxidase reagents are destroyed by sodium azide and should be avoided in all buffers and regents. 11. Wash slide with deionized or distilled for 2-3X. 12. Incubate with AEC or DAB chromogen reagent. 13. Wash 5-7X with buffer. 14. Incubate with counterstain. 15. Wash slide with tap water distilled water. 16. Mount slide with appropriate mounting medium.

关键属性

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储存与操作

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储存2-8 °C, Do Not Freeze
有效期12 months from date of receipt.
免责声明For research use only

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质量保证

质量保证

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实验方案信息

IHC
免疫组化
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ICC
免疫细胞化学
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可选规格

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15 ml
¥ 6,500.00
发货Usually dispatched within 5-10 working days
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