pCp-Biotin

SKU: orb178353

描述

pCp-Biotin enables 3'-end labeling of RNA/miRNA using T4 RNA Ligase. These probes support hybridization experiments like microarrays or EMSA. Detection utilizes streptavidin-conjugated HRP, AP, or dyes. Alternatively, streptavidin beads can purify labeled probes for further analysis.

图片与验证

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应用说明	Incorporation into RNA/miRNA by 3'-End Labeling with T4 RNA Ligase. Spectroscopic Propertie: λmax 271 nm, ε 8.9 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

关键属性

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MW	Theoretical MW: 728.65 g/mol (free acid); Detected MW: 728.20 g/mol (free acid)
纯度	≥ 95% (HPLC)
分子式	C₂₅H₄₂N₆O₁₃P₂S₁
SMILES	Nc1nc(=O)n(cc1)[C@@H]1O[C@H](COP(=O)(O)O)[C@H]([C@H]1O)OP(=O)(OCCCCCCNC(=O)CCCC[C@@H]1SC[C@H]2[C@@H]1NC(=O)N2)O

客户验证数据

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以下数据由各地研究人员提交。即使我们尚未自行检测，这些结果也有助于判断该产品是否适合您的应用或种属。

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反应性分布

最新实验

7 Results

稀释

样本

摘要

RNA imaging

Mouse

In vitro-induced plasma cell (iPC)

IPC cells were UV-crosslinked and lysed,...

TRIC - sequencing

Bacteria

Stationary-phase cultures of Escherichia coli, Stutzerimonas stutzeri, Staphylococcus aureus, and Myxococcus xanthus.

pCp-Biotin is used to label the...

Northern blot

Virus

Cucumis sativus) plants infected with ASSVd

pCp-Biotin is used to enzymatically label...

RNA labeling

Human

Human HEK-293 cells expressing Myc-tagged TTP variants

pCp-Biotin (pOp-Biotin) is used for the...

RNA labeling

Human

Human monocytic cell line THP-1

pCp-Biotin is used in a bulk...

储存与操作

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储存	store at -20 °C
有效期	12 months from date of receipt.
危害信息	Non-Toxic
免责声明	For research use only

别名

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Cytidine-5-phosphate-3-(6-aminohexyl)phosphate, labeled with Biotin, Triethylammonium salt

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

化合物标识

PubChem CID

168436663 ↗

关键属性

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暂无计算属性。

实验方案信息

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pCp-Biotin

描述

图片与验证

关键属性

客户验证数据

按应用统计成功数

反应性分布

最新实验

储存与操作

别名

质量保证

化合物标识

关键属性

实验方案信息

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