pCp-Desthiobiotin

SKU: orb533108

描述

pCp-Desthiobiotin is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single Desthiobiotin molecule to the 3'-OH group of the RNA/miRNA template. The resulting Desthiobiotin-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA). Detection of the Desthiobiotin moiety can be performed using streptavidin con-jugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. Alternatively, Desthiobiotin-labeled RNA/miRNA probes can also be purified with Streptavidin-conjugated agarose/magnetic beads.

图片与验证

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应用说明	Incorporation into RNA/miRNA by 3'-End Labeling with T4 RNA Ligase. Spectroscopic Propertie: λmax 271 nm, ε 8.9 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

关键属性

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MW	Theoretical MW: 698.60 g/mol (free acid); Detected MW: 698.24 g/mol (free acid)
纯度	≥ 95% (HPLC)
分子式	C₂₅H₄₄N₆O₁₃P₂
SMILES	Nc1nc(=O)n(cc1)[C@@H]1O[C@H](COP(=O)(O)O)[C@H]([C@H]1O)OP(=O)(OCCCCCCNC(=O)CCCCC[C@H]1C(NC(=O)N1)C)O

储存与操作

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储存	store at -20 °C
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Cytidine-5'-phosphate-3'-(6-aminohexyl)phosphate, labeled with Desthiobiotin, Triethylammonium salt

质量保证

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化合物标识

PubChem CID

176417489 ↗

关键属性

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暂无计算属性。

实验方案信息

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pCp-Desthiobiotin

描述

图片与验证

关键属性

储存与操作

别名

质量保证

化合物标识

关键属性

实验方案信息

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