PECAM-1, Mouse, mAb MEC7.46 Antibody

SKU: orb1109192

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描述

Mouse monoclonal antibody to PECAM-1

研究领域

Immunology & Inflammation

图片与验证

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已测应用	FC, IF, IHC-Fr, IP, PS
稀释范围	Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
反应性	Mouse
应用说明	FC: Antibody MEC7.46 stains the extracellular domain of mouse PECAM-1; 106 cells were used per sample. No fixation or permeabilization needed. Epitope is sensitive to trypsin! (Ref.1) F: Tissue sections were fixed in acetone for 10 min at RT and pretreated with 0.01% hydrogen peroxide to quench endogenous peroxidases. Normal rabbit serum was used as blocking agent. As (Ref.1). P: Serial paraffin-embedded 4 um sections were stained by H&E, PAS and Azan Histochemistry. The distribution of endothelial (Mec7.46), matrix (laminin) and macrophage (FA/11) markers was evaluated using a standard three-step ABC method and development in 3-amino-9-ethylcarbazole solution. Sections were counterstained for 45 seconds with Harris' haematoxylin at room temperature and mounted in Imsol-mount medium (ref. 3). IP: Lysate of 35S-Methionine labeled 5HV cells was used. Molecule of ~130 kDa.

关键属性

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克隆性	Monoclonal
同种型	Rat IgG1
免疫原	Mouse t.end.1 cells (polyoma middle T (PmT)-transformed EC)
偶联	Unconjugated

储存与操作

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储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
形态/外观	1 ml (100 µg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
缓冲液/防腐剂	0.2 ml (100 μg/ml) 0.2 μm filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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CD31, platelet-endothelial cell adhesion molecule-1 Gene name: Pecam1

质量保证

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