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描述
图片与验证
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Item 1 of 9
| 已测应用 | ELISA, IF, IHC, WB |
|---|---|
| 稀释范围 | ELISA: 1:8,000 - 1:32,000, IHC: 1:100-1:300, WB: 1:500 - 1:2,000 |
| 反应性 | Human, Mouse |
| 应用说明 |
关键属性
−| 抗体类型 | Primary Antibody |
|---|---|
| 宿主 | Rabbit |
| 克隆性 | Polyclonal |
| 同种型 | IgG |
| 免疫原 | PPAR alpha Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near amino acids 1-25 of mouse PPAR alpha. |
| 靶点 | Ppara |
| 纯度 | This affinity purified antibody is directed against mouse PPAR alpha protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, rat, bovine, dog, golden hamster and boar sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR alpha protein from human, chimpanzee and rhesus monkey may also occur as this sequence shows 88% homology (16/18 identities) with the protein from these sources. Cross reactivity with PPAR alpha homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR. |
| 偶联 | Unconjugated |
储存与操作
−| 储存 | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| 形态/外观 | Liquid (sterile filtered) |
| 缓冲液/防腐剂 | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| 浓度 | 1.0 mg/mL |
| 有效期 | 12 months from date of receipt. |
| 干冰运输 | 请注意：本产品需要干冰运输，将收取干冰附加费。 |
| 免责声明 | For research use only |
别名
−rabbit anti-Ppar alpha antibody, Pparα, Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1, Ppar-a, Nr1c1, Ppar
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质量保证
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