Puromycin 2HCl

SKU: orb1306074

特色

描述

Puromycin dihydrochloride is a protein synthesis inhibitor that acts by binding to RNA. This cinnamamide adenosine antibiotic is utilized in biochemical research for its antitumor and antitrypanosomal properties, facilitating studies in both in vitro and in vivo experimental models.

研究领域

Cell Biology, Neuroscience

图片与验证

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关键属性

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CAS 号	58-58-2
MW	544.43
纯度	>98%
分子式	C₂₂H₂₉N₇O₅ ∙ 2HCl
SMILES	Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@@H]2[C@@H](CO)O[C@H]([C@@H]2O)n2cnc3c(ncnc23)N(C)C)cc1
靶点	Ribosome
溶解性	Soluble in DMSO (up to 50 mg/ml), or in Water (up to 50 mg/ml).

生物活性

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靶点 IC50	MOLT-4 cells:0.17 μM (EC50)\|KB-3-1 cells:0.21 μM\|PRH cells:2000 nM\|HEK293 cells:0.35 nM\|J774.A1 cells:10 μM\|HEK293T cells:0.42 μM (CC50)\|K562 cells:0.22 μM\|A549 cells:300 nM\|HepG2 cells:1600 μM\|KB-V1 cells:79.3 μM
体内	Puromycin is utilized to select recombinant cells from non-cultured cells. It serves as a tool for studying protein synthesis in many systems.
体外	METHODS: Human hepatocellular carcinoma cells, HepG2, and primary rat hepatocytes, PRH, were treated with Puromycin dihydrochloride (0-300 µmol/L) for 72 h, and cell growth inhibition was detected by MTT. RESULTS: Puromycin dihydrochloride dose-dependently inhibited the growth of HepG2 and PRH cells, with IC50s of 1600 μmol/L and 2000 nmol/L, respectively. METHODS: Human breast cancer cells MDA-MB-231 and MDA-MB-436 were transfected with shRNAs-Puro lentivirus, and after 24 h, the transfected cells were cultured in new medium containing Puromycin dihydrochloride (1 μg/ml) for about 12 days, and then the cells were screened for the success of transfection. RESULTS: Puromycin dihydrochloride screened the lentivirally transfected cells successfully. METHODS: Cells transfected with lentivirus were cultured in medium supplemented with Puromycin dihydrochloride (2 μg/mL). RESULTS: Puromycin dihydrochloride screened and maintained lentivirally successfully transfected cells.

储存与操作

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储存	-20°C
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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CL-13900, CL13900 dihydrochloride, CL13900 Dihydrochloride, CL-13900 Dihydrochloride, CL 13900, CL 13900 Dihydrochloride, CL13900, DNASynthesis, DNA Synthesis, DNA synthesis, 60S ribosome, Bacterial, Antibiotic, inhibit, Inhibitor, Puromycin, Puromycin 2HCl, Puromycin Dihydrochloride, Puromycin dihydrochloride, RNASynthesis, RNA Synthesis

相似产品

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Puromycin 2HCl [orb3066714]
可选规格： 50 mg, 250 mg

质量保证

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化合物标识

PubChem CID

443311 ↗

关键属性

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暂无计算属性。

实验方案信息

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Puromycin 2HCl

描述

研究领域

图片与验证

关键属性

生物活性

储存与操作

别名

相似产品

Puromycin 2HCl [orb3066714]

质量保证

化合物标识

关键属性

实验方案信息

可选规格