Rabbit anti Goat IgG (heavy and light chains) (non Human and Mouse) Antibody (FITC)

SKU: orb21499

描述

Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to goat IgG, heavy and light chains, adsorbed for cross-reactivity with human and mouse immunoglobulins. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immuno- fluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of goat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

研究领域

Immunology & Inflammation; Cell Biology

图片与验证

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反应性	Goat

关键属性

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抗体类型	Secondary Antibody
宿主	Rabbit
克隆性	Polyclonal
偶联	FITC

储存与操作

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储存	The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
形态/外观	FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added
有效期	12 months from date of receipt.
免责声明	For research use only

质量保证

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实验方案信息

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