Rabbit anti Mouse IgG1 IgG2a IgG2b IgG3 IgA IgM (heavy and light chains) (non Human) Antibody (FITC)

SKU: orb22092

描述

Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to Mouse IgG, IgA and IgM, heavy and light chains, adsorbed for human cross-reactivity. Direct immunofluorescence staining of monoclonal mouse monoclonal antibodies in the presence of human cell or serum components at the cellular and subcellular level. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.

研究领域

Immunology & Inflammation

图片与验证

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反应性	Mouse

关键属性

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抗体类型	Secondary Antibody
宿主	Rabbit
克隆性	Polyclonal
偶联	FITC

储存与操作

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储存	The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
形态/外观	FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
有效期	12 months from date of receipt.
免责声明	For research use only

质量保证

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实验方案信息

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