Rapamycin

SKU: orb1308282

特色

描述

Rapamycin is a natural macrolide and potent mTOR inhibitor, demonstrating high specificity with an IC50 of 0.1 nM in HEK293 cells. This compound is widely used in research for its immunosuppressive properties and ability to induce autophagy, applicable in both in vitro mechanistic studies and in vivo models of aging and disease.

研究领域

Cell Biology, Immunology & Inflammation

图片与验证

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关键属性

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CAS 号	53123-88-9
MW	914.2
纯度	>98%
分子式	C₅₁H₇₉NO₁₃
SMILES	CO[C@@H]1C[C@H](C[C@@H](C)[C@@H]2CC(=O)[C@H](C)\C=C(C)\[C@@H](O)[C@@H](OC)C(=O)[C@H](C)C[C@H](C)\C=C\C=C\C=C(C)\[C@H](C[C@@H]3CC[C@@H](C)[C@@](O)(O3)C(=O)C(=O)N3CCCC[C@H]3C(=O)O2)OC)CC[C@H]1O
靶点	Kinase
溶解性	Soluble in DMSO (up to 40 mg/ml) or in Ethanol (up to 90 mg/ml).

客户验证数据

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以下数据由各地研究人员提交。即使我们尚未自行检测，这些结果也有助于判断该产品是否适合您的应用或种属。

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反应性分布

最新实验

1 Results

稀释

样本

摘要

In vivo

Mouse

TM4 Sertoli cells

For rapamycin (RAPA), an autophagy activator,...

生物活性

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靶点 IC50	MCF-7 cells:18.74 μM\|MDA-MB-468 cells:37.2 μM\|mTOR:0.1 nM (HEK293 cells)\|A549 cells:18.74 μM\|HeLa cells:> 10000 nM\|MEF cells:0.05 nM\|MDA-MB-231 cells:> 10000 nM\|HEK293T cells:0.1 nM (EC50)\|HEK293 cells:0.1 nM
体内	METHODS: To study the effect of Rapamycin on life expectancy, Rapamycin (8 mg/kg in DMSO+5% PEG-400+5% Tween-80) was administered intraperitoneally to 20-21 month old C57BL/6J mice once daily for three months. RESULTS: Three months of Rapamycin treatment was sufficient to increase the life expectancy of middle-aged mice by 60% and improve their healthy lifespan. METHODS: To determine the appropriate dose of Rapamycin for the treatment of epilepsy, Rapamycin (0.1-3 mg/kg in 4% ethanol + 5% Tween 80 + 5% PEG 400) was injected intraperitoneally into Sprague-Dawley rats once a day for four weeks. RESULTS: Only 1.0 mg/kg and 3.0 mg/kg Rapamycin inhibited p-S6. Rats treated with 0.1 and 0.3 mg/kg Rapamycin had no significant adverse effects, whereas rats treated with 1.0 and 3.0 mg/kg Rapamycin showed significant reductions in body, spleen, and thymus weights, and exhibited cognitive impairment and anxiety. The Rapamycin dose could not inhibit mTOR in the treatment of epilepsy without causing any side effects, but 1 mg/kg may be the optimal dose to inhibit mTOR in young rats with relatively few side effects.
体外	METHODS: Normal human renal epithelial cells HRECs were treated with Rapamycin (0.01-1000 nmol/L) for 6 days, and cell growth inhibition was detected using MTT. RESULTS: Rapamycin dose-dependently inhibited the growth of HRECs, with a 40% reduction in cell viability at a concentration of 10 nmol/L. METHODS: Human cervical cancer cells HeLa and human prostate cancer cells PC3 were treated with Rapamycin (100 nM) for 0.5-24 h, and the expression levels of target proteins were detected by Immunoprecipitation. RESULTS: Rapamycin had little effect on the expression levels of mTOR, raptor and rictor. Rapamycin significantly reduced raptor binding to mTOR at 0.5 h and rictor binding to mTOR at 24 h. Long-term treatment of cells with Rapamycin inhibited mTORC2 assembly. METHODS: Human vascular endothelial cells were treated with Rapamycin (1-100 ng/mL) for 48 h, and cell migration was examined using the Wound-healing method. RESULTS: Rapamycin dose-dependently inhibited the migration of human vascular endothelial cells.
细胞研究	To determine the effects of rapamycin and rapamycin plus LY294002 or UCN-01 on tumor cells, we determined cell viability after the treatments. We used a trypan blue dye exclusion assay as described previously. Tumor cells in exponential growth were harvested and seeded at 5 × 10^3 cells per well (0.1 mL) in 96-well flat-bottomed plates and incubated overnight at 37°C. The cells were then incubated for 72 hours with or without rapamycin or with rapamycin plus LY294002 or UCN-01. After the cells were collected by trypsinization, they were stained with trypan blue, and the viable cells in each well were counted. The viability of the untreated cells (the control) was considered 100%. Survival fractions were calculated from the mean cell viability of the treated cells .
动物研究	Animals were randomized to treatment or vehicle groups so that the mean starting body weights of each group were equal. Drug treatment began on the day of surgery or on the first day of reloading after the 14-day suspension. Rapamycin was delivered once daily by intraperitoneal injection at a dose of 1.5 mg/kg, dissolved in 2% carboxymethylcellulose. CsA was delivered once daily by subcutaneous injection at a dose of 15 mg/kg, dissolved in 10% methanol and olive oil. FK506 was delivered once daily via subcutaneous injection at a dose of 3 mg kg 1, dissolved in 10% ethanol, 10% cremophor and saline .

储存与操作

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储存	-20°C
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Sirolimus; AY-22989; WY-090217; NSC-226080; Rapamune

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质量保证

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化合物标识

PubChem CID

5284616 ↗

关键属性

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暂无计算属性。

实验方案信息

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应用：	ELISA, ICC, IHC, WB
反应性：	Human, Mouse, Rat
种属/宿主：	Rabbit
克隆性：	Polyclonal
偶联：	Unconjugated

应用：	ELISA, ICC, IF, IHC
反应性：	Human, Mouse, Rat
种属/宿主：	Rabbit
克隆性：	Polyclonal
偶联：	Unconjugated

应用：	ELISA, FC, ICC, IF, IHC, WB
反应性：	Human, Mouse, Rat
种属/宿主：	Rabbit
克隆性：	Polyclonal
偶联：	Unconjugated

Rapamycin

描述

研究领域

图片与验证

关键属性

客户验证数据

按应用统计成功数

反应性分布

最新实验

生物活性

储存与操作

别名

相似产品

mTOR Rabbit Polyclonal Antibody [orb3160046]

MTOR Rabbit Polyclonal Antibody [orb13590]

Phospho-mTOR (Ser2481) Rabbit Polyclonal Antibody [orb6453]

RPTOR Rabbit Polyclonal Antibody [orb3161512]

MLST8 Rabbit Polyclonal Antibody [orb1786046]

质量保证

化合物标识

关键属性

实验方案信息

可选规格