Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
关键属性
−
抗体类型
Secondary Antibody
克隆性
Monoclonal
同种型
Rat IgG2a
克隆号
RTG2A1-1
纯化
Purified by protein-G affinity chromatography.
偶联
Unconjugated
储存与操作
−
储存
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂
Phosphate buffered saline (PBS), pH 7.4
浓度
1 mg/ml
有效期
12 months from date of receipt.
免责声明
For research use only
质量保证
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