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Rat IgG2a Isotype Control Monoclonal Antibody (PE)

SKU: orb1293831
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描述

The Rat IgG2a Isotype Control-PE labeled Antibody is suitable for FC, IF. It is a Monoclonal, PE conjugated antibody. Purification: The antibody was purified by affinity chromatography.

图片与验证

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Rat IgG2a Isotype Control Monoclonal Antibody (PE)
已测应用FC, IF
稀释范围10 μl / assay
应用说明
1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

关键属性

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抗体类型Primary Antibody
宿主Rat
克隆性Monoclonal
同种型Rat IgG2a
克隆号B4C5
纯化The antibody was purified by affinity chromatography.
偶联PE

储存与操作

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储存Shipped and store at 4°C for one year. Do not freeze.
形态/外观Liquid
缓冲液/防腐剂PBS, pH 7.3, and 0.02% sodium azide.
有效期12 months from date of receipt.
免责声明For research use only

相似产品

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质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

实验方案信息

FC
流式细胞术
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IF
免疫荧光
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可选规格

请在下方选择规格

25 assay
¥ 1,690.00
100 assay
¥ 3,120.00
免费二抗（20 µl）0/0

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发货Usually dispatched within 5-10 working days
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