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Rat IgG2b Isotype Control Monoclonal Antibody

SKU: orb1293407
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描述

The Rat IgG2b Isotype Control Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody .Purification: The antibody was purified by affinity chromatography.

图片与验证

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Rat IgG2b Isotype Control Monoclonal Antibody
已测应用FC, IF
稀释范围IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
应用说明
1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-rat IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

关键属性

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抗体类型Primary Antibody
宿主Rat
克隆性Monoclonal
同种型Rat IgG2b
克隆号B3X4
纯化The antibody was purified by affinity chromatography.
偶联Unconjugated

储存与操作

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储存Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
形态/外观Liquid
缓冲液/防腐剂PBS, pH 7.3, and 0.02% sodium azide.
有效期12 months from date of receipt.
免责声明For research use only

相似产品

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质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

实验方案信息

FC
流式细胞术
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IF
免疫荧光
查看实验方案
查看更多实验方案

可选规格

请在下方选择规格

30 μl
¥ 1,820.00
50 μl
¥ 2,340.00
100 μl
¥ 3,510.00
200 μl
¥ 4,810.00
选择偶联或无载体版本
免费二抗（20 µl）0/0

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发货Usually dispatched within 5-10 working days
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