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描述
研究领域
Immunology & Inflammation
图片与验证
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关键属性
−| 表达系统 | E.coli |
|---|---|
| 生物学来源 | Human |
| 生物学活性 | ELISA: Immobilized Recombinant Human CD40 (C-Fc)(Cat#orb2994010) at 2μg/ml (100 μl/well) can bind Recombinant Human CD40L(Cat#orb2995248)*.*: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Human CD40L(Cat#orb2995248) is 1-10 ng/ml. (QC verified). BLI: Loaded Human CD40-His (Cat#orb2995146) on HIS1K Biosensor, can bind Human CD40L (Cat#orb2995248) with an affinity constant of 2.43 nM as determined in BLI assay. (Regularly tested) Loaded Anti-Human CD40L mAb-Fc(Cat#orb2993151) on Protein A Biosensor, can bind Human CD40L(Cat#orb2995248) with an affinity constant of 0.05 nM as determined in BLI assay. |
| 标签 | Tag free |
| 表达区域 | Met113-Leu261 |
| 内毒素 | ＜1EU/μg as determined by LAL test. |
| MW | Predicted: 16.2 kDa. Observed: 15 kDa, reducing conditions |
| 纯度 | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
储存与操作
−| 储存 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| 形态/外观 | Lyophilized |
| 缓冲液/防腐剂 | 0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 有效期 | 6 months from date of receipt. |
| 免责声明 | For research use only |
别名
−CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP
相似产品
−- Item 1 of 4
CD40/TNFRSF5 Human Recombinant Antibody [orb2958459]
ELISA, FC, WB
Human
Human
Monoclonal
Unconjugated
100 μg, 1 mg, 50 μg - Item 1 of 1
Recombinant Mouse CD40 Protein [orb2994384]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 46.5 KDa. Observed: 50-58 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human CD40L Protein [orb2994532]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Predicted: 16.2 KDa. Observed: 16-18 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Cynomolgus CD40 Protein [orb2994786]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 46.3 KDa. Observed: 48-70 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg
质量保证
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.
常用数据库链接
UniProt
UniProt 详情
−暂无 UniProt 数据
按种属筛选
Huey-Liang Kuo, Chiu-Ching Huang, Tze-Yi Lin, Ching-Yuang Lin IL-17 and CD40 ligand synergistically stimulate the chronicity of diabetic nephropathy Nephrology Dialysis Transplantation, 33, 248 (2018)
反应性
Human
