Recombinant S100A4 Antibody / FSP1

SKU: orb2642282

描述

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A4 may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [RefSeq]

研究领域

Signal Transduction

图片与验证

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已测应用	FACS, IHC-P, WB
稀释范围	Flow cytometry: 1-2ug/10^6 cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
反应性	Human
应用说明	The stated application concentrations are suggested starting points. Titration of the S100A4 antibody may be required due to differences in protocols and secondary/substrate sensitivity. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型	Primary Antibody
宿主	Rabbit
克隆性	Recombinant
同种型	Rabbit IgG, kappa
克隆号	PS1A4-1R
免疫原	A portion of amino acids 1-200 from the human protein was used as the immunogen for this recombinant S100A4 antibody.
纯化	Protein A affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
有效期	12 months from date of receipt.
免责声明	For research use only

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质量保证

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