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SAG

SKU: orb1307461

描述

SAG

研究领域

Pharmacology & Drug Discovery, Stem Cell & Developmental Biology

图片与验证

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关键属性

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CAS 号912545-86-9
MW490.06
纯度98.88% (May vary between batches)
分子式C28H28ClN3OS
SMILESCN[C@H]1CC[C@@H](CC1)N(Cc1cccc(c1)-c1ccncc1)C(=O)c1sc2ccccc2c1Cl
靶点Smo,Hedgehog/Smoothened
溶解性DMSO:252.5 mg/mL (515.24 mM);Ethanol:40 mg/mL (81.62 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5.5 mg/mL (11.22 mM)

生物活性

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靶点 IC50
C3H 10T1/2 cells:0.13 μM (EC50)|Shh Light II cells:8.8 nM (EC50)|Smo:3 nM (EC50)|HUVECs:22 μM
体内
METHODS: To explore whether the SHH signaling pathway plays a protective role in anxiety by regulating mitochondrial homeostasis, SAG (10 mg/kg) was intraperitoneally injected into C57BL/6 mice on a high-fat diet every three days for twelve weeks. RESULTS: SHH signaling is neuroprotective in obesity, and SAG alleviates anxiety-like behaviors by reducing mitochondrial breaks. METHODS: To study the effects on developing limbs, SAG (15-20 mg/kg in lactated Ringer's solution) was administered as a single intraperitoneal injection to pregnant C57BL/6J mice at gestational day (GD) 9.25. RESULTS: The most prevalent effect of SAG was the dose-dependent induction of preaxial polydactyly; defects ranged from thumb width to duplication of both digitiform fingers on the preaxial side of the thumb.
体外
METHODS: Embryonic stem cell ES were treated with SAG (0.5-1.25 µM) for 4-6 days and gene expression levels were measured using RT-qPCR. RESULTS: Expression of Sim1, a post-mitotic V3 marker, was increased in all SAG-treated groups at all concentrations. The group treated with 0.5 μM SAG exhibited significantly higher levels of Nkx2.2 mRNA. Hb9 expression did not change significantly with SAG concentration at the given time points. METHODS: African green monkey kidney fibroblast-like cells, Cos-1, were treated with SAG (5-500 nM), and target protein expression levels were measured by Western Blot. RESULTS: SAG inhibited cross-linking of ER-localized and post-ER forms of Smo-Myc3 to 125I-labeled PA cyclic amines in Cos-1 cells in a dose-dependent manner. cellular levels of Smo-Myc3 were not affected by agonist treatment.

储存与操作

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储存-20°C
有效期12 months from date of receipt.
免责声明For research use only

别名

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SAG, Smoothened Agonist, Smoothened, Smo, Hedgehog

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关键属性

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实验方案信息

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1 mg
¥ 1,170.00
2 mg
¥ 1,430.00
5 mg
¥ 1,690.00
1 ml x 10 mM (in DMSO)
¥ 1,690.00
10 mg
¥ 2,210.00
25 mg
¥ 3,770.00
50 mg
¥ 4,940.00
100 mg
¥ 7,150.00
200 mg
¥ 9,880.00
发货Usually dispatched within 5-10 working days
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