SC-79

SKU: orb1305828

特色

描述

SC79 is a blood-brain barrier permeable small molecule that acts as a specific AKT agonist by binding to its PH domain. It activates cytoplasmic AKT while inhibiting its membrane translocation, demonstrating neuroprotective effects in both cellular and animal models of neurological injury.

研究领域

Neuroscience

图片与验证

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关键属性

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CAS 号	305834-79-1
MW	364.79
纯度	>98%
分子式	C₁₇H₁₇ClN₂O₅
SMILES	CCOC(=O)C(C#N)C1C(C(=O)OCC)=C(N)Oc2ccc(Cl)cc12
靶点	Kinase
溶解性	Soluble in DMSO (up to 100 mg/ml) or in Ethanol (up to 40 mg/ml with warming).

生物活性

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靶点 IC50	Akt:4 µg/mL
体内	METHODS: To detect in vivo activity, SC79 (0.04 mg/g) was injected intraperitoneally into a C57 Black/6 mouse model of copper relaxation demyelination, and 5 min later middle cerebral artery occlusion (MCAO) was performed to construct an ischemic stroke model. RESULTS: A single dose of SC79 reduced the size of neocortical lesions by more than 35% and 40% at 24 h after MCAO and 1 week after MCAO, respectively. METHODS: To investigate the effect on liver injury, SC79 (10 mg/kg) was injected intraperitoneally into C57BL/6 mice, and d-Gal/LPS was injected 0.5 h later to induce liver injury. RESULTS: SC79 protected mice from TNF-α-mediated liver injury induced by d-Gal/LPS.
体外	METHODS: Human cervical cancer cells were starved of HeLa serum for 1 h, treated with SC79 (4 µg/mL) for 30 min, and the expression levels of target proteins were detected by Western Blot. RESULTS: SC79 enhanced AKT phosphorylation, and SC79-induced AKT phosphorylation mainly occurred in the cytoplasm. METHODS: Human lung cancer cells A549 were treated with SC79 (10 µg/mL) for 24 h. The gene expression level was detected by qPCR. RESULTS: SC79 treatment up-regulated the expression of Nrf-2 (NFE2L2) gene itself as well as the downstream targets HO-1 and NQO-1.
细胞研究	HsSultan or NB4 cells (2.5 × 105) are plated in a 24-well plate in 500 μL of phenol red-free RPMI medium supplemented with 10% FBS. After incubation for 24 hours, each compound (8 μg/mL) is added and cultured for overnight (16–20 h). Fifty microliters of MTT solution (5 mg/mL in PBS) are added to each well. Following 2 hrs incubation, the purple formazan crystals are dissolved by directly adding in 500 μL of isopropanol with 0.1 M HCl to each well. After clearing the cell debris by centrifugation, the absorbance is measured at a wavelength of 570 nm.(Only for Reference)

储存与操作

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储存	-20°C
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Akt, Inhibitor, inhibit, SC 79, SC79, SC-79, PKB, Protein kinase B

相似产品

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SC-79 [orb1225359]
纯度：
>98% (HPLC)
CAS 号：
305834-79-1
MW:
364.7803
分子式：
C₁₇H₁₇ClN₂O₅
可选规格： 5 mg, 10 mg, 25 mg, 100 mg, 200 mg, 50 mg, 500 mg, 1 g

质量保证

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化合物标识

PubChem CID

2810830 ↗

关键属性

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暂无计算属性。

实验方案信息

查看更多实验方案

纯度：	>98% (HPLC)
CAS 号：	305834-79-1
MW:	364.7803
分子式：	C₁₇H₁₇ClN₂O₅

SC-79

描述

研究领域

图片与验证

关键属性

生物活性

储存与操作

别名

相似产品

SC-79 [orb1225359]

质量保证

化合物标识

关键属性

实验方案信息

可选规格