Sheep anti Human IgD (Fc specific) Antibody (TRITC)

SKU: orb21570

描述

Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal sheep antiserum to human IgD, Fc specific. Direct immunofluorescence staining of cytoplasmic IgD of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:30.

研究领域

Immunology & Inflammation

图片与验证

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反应性	Human

关键属性

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抗体类型	Secondary Antibody
宿主	Sheep
克隆性	Polyclonal
偶联	TRITC

储存与操作

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储存	The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
形态/外观	TRITC-coupled purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered No preservative added, as it may interfere with the antibody activity
有效期	12 months from date of receipt.
免责声明	For research use only

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