SR-9009

SKU: orb1303901

特色

描述

SR9009 is a potent REV-ERBα/β agonist used in circadian rhythm and metabolism research. It alters core clock gene expression and increases energy expenditure. In vivo studies demonstrate its effects in reducing adiposity and improving metabolic parameters in models of diet-induced obesity.

研究领域

Cell Biology

图片与验证

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关键属性

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CAS 号	1379686-30-2
MW	474.40
纯度	>98%
分子式	C₂₀H₂₄CLN₃O₄S · HCl
SMILES	CCOC(=O)N1CCC(CN(Cc2ccc(s2)[N+]([O-])=O)Cc2ccc(Cl)cc2)C1
靶点	Transcription factor
溶解性	Soluble in DMSO (greater than 25 mg/ml) or in Ethanol (up to 20 mg/ml).

生物活性

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靶点 IC50	Rev-Erbα:670 nM\|Rev-Erbβ:800 nM
体内	SR9009 inhibits the activity of the SCN clock, with reversible inhibition of circadian oscillations in SCN explants cultured from the Per2: Luc reporter mouse.
体外	SR9009 effectively inhibits transcription in a cotransfection assay using full-length REV-ERBα along with a luciferase reporter driven by the Bmal1 promoter (SR9009 IC50: 710 nM). SR9009 suppresses the expression of BMAL1 mRNA in HepG2 cells in a REV-ERBα/β-dependent manner.
细胞研究	SR9009 is dissolved in DMSO and diluted with appropriate media. HEK293 cells are grown in 96-well plates (1×106/well) and are transiently transfected using Lipofectamine. Cells are transfected with a total of 200 ng of DNA per well consisting of the pGL4 mIL-17 firefly luciferase reporter construct, the pGL4 mIL-17 + CNS-5 firefly luciferase reporter construct, or the pGL4 mIL-17 2kB RORE mutant (100 ng/well) , an actin promoter Renilla reniformis luciferase reporter (50 ng/well), and either control vector alone or the test DNA (full-length RORα or full-length RORγ at 50 ng/well). All 48 human nuclear receptors are represented in the specificity assay and SR9009 is tested at a concentration of 20 μM. The format of the assay is a cotransfection assay with Gal4 DNA binding domain-nuclear receptor fusions in HEK293 cells.

储存与操作

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储存	-20°C
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Autophagy, inhibit, Inhibitor, SR 9009, SR9009, SR-9009, Stenabolic, Rev-ErbBα, Rev-ErbBβ, REV-ERB Agonist II

相似产品

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SR9009 [orb1223325]
纯度：
>98% (HPLC)
CAS 号：
1379686-30-2
MW:
437.94
分子式：
C₂₀H₂₄ClN₃O₄S
可选规格： 2 mg, 5 mg, 10 mg, 25 mg, 50 mg, 100 mg, 500 mg, 1 g

质量保证

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化合物标识

PubChem CID

57394020 ↗

关键属性

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暂无计算属性。

实验方案信息

查看更多实验方案

纯度：	>98% (HPLC)
CAS 号：	1379686-30-2
MW:	437.94
分子式：	C₂₀H₂₄ClN₃O₄S

SR-9009

描述

研究领域

图片与验证

关键属性

生物活性

储存与操作

别名

相似产品

SR9009 [orb1223325]

质量保证

化合物标识

关键属性

实验方案信息

可选规格