Swine Annexin V-Fluorescein Apoptosis Assay Kit

SKU: orb1409909

描述

For quickly and easily distinguishing two populations of dying cells from viable cells using recombinant fluorescein-conjugated Swine Annexin V and Propidium Iodide. Results can be analyzed via flow cytometer.

研究领域

Cell Biology

图片与验证

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应用说明

1. Prepare the standard and samples in the Sample and Standard Diluent. 2. Add 50 µL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 µL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour. 5. Dilute 10X Wash Buffer 1:10 with diH2O. 6. Wash plate 4 times with 1X Wash Buffer. 7. Add 100 µL TMB Substrate to each well. 8. Cover plate with Plate Cover and incubate at room temperature in the dark for 30 minutes to develop the signal. 9. Add 100 µL Stop Solution to each well. 10. Measure absorbance using a plate reader at 450 nm. 11. Plot the standard curve and calculate sample concentrations.

关键属性

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样本类型	Cell culture

储存与操作

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储存	2-8°C
有效期	6 months from date of receipt.
免责声明	For research use only

相似产品

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Swine Annexin V-Fluorescein Apoptosis Assay Kit [orb746825]
可选规格： 500 T

质量保证

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实验方案信息

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