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tdTomato Antibody

SKU: orb182397

描述

Goat polyclonal antibody to tdTomato (red fluorescent protein). tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation.

图片与验证

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已测应用ELISA, FACS, IF, IHC-Fr, IHC-P, WB
稀释范围WB 1:500-1:2,000 IHC (F) 1:50-1:500 IHC (P) 1:50-1:500 IF 1:50-1:500
反应性Other
应用说明
In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein)

关键属性

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宿主Goat
克隆性Polyclonal
同种型IgG
免疫原Antigen: Purified recombinant peptide produced in E. coli. Antigen Sequence: MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK
靶点Red Fluorescent Protein
蛋白序列MEQKLISEEDLGTISTMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSNGGMDEVYK
分子量55 kDa
纯度This antibody is epitope-affinity purified from goat antiserum
纯化Epitope affinity purified
偶联Unconjugated

客户验证数据

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以下数据由各地研究人员提交。即使我们尚未自行检测,这些结果也有助于判断该产品是否适合您的应用或种属。
按应用统计成功数
反应性分布

最新实验

5 Results
稀释
样本
摘要
Flow cytometry
Mice
1:200
Transfected mice
Following fixation, cells were washed thoroughly...
IF
Drosophila
1:200
Adult Drosophila melanogaster brains
Fly brains were dissected, fixed, and...
IHC
Mice
1:600
Mice brain tissue
Preparation involved perfusion fixation, sectioning, and...
ICC
Human
1:300
Human pluripotent stem cells (hPSC)
Sample preparation involved fixing seeded scaffolds...
WB
Rat
-
Rat PC12 or primary astrocyte cells
PC12 or primary astrocyte cells were...

储存与操作

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储存For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours
形态/外观Polyclonal antibody supplied as a 200 or 500 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
浓度1 mg/ml
有效期12 months from date of receipt.
免责声明For research use only

别名

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Cherry fluorescent protein, DsRed, mCherry, red fluorescent protein, RFP antibody

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质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

实验方案信息

WB
免疫印迹(WB)
查看实验方案
IHC-P
石蜡切片免疫组化
查看实验方案
IHC-Fr
冰冻切片免疫组化
查看实验方案
FACS
荧光激活细胞分选(FACS,流式细胞术)
查看实验方案
IF
免疫荧光
查看实验方案
ELISA
酶联免疫吸附测定(ELISA)
查看实验方案

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IF

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IF

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IF

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应用

IF, IHC

反应性

Mouse

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应用

IF

反应性

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应用

IF

反应性

Rat

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100 μg
¥ 3,640.00
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