2 × Phanta Flash Master Mix is a new generation superior enzyme based on Phanta Flash Super-Fidelity DNA Polymerase. Throughdirected optimization of Phanta DNA Polymerase, Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of rapid amplification (4 - 5 sec/kb) while maintaining high fidelity and yield. The amplification error rate of Phanta Flash Super-Fidelity DNA Polymerase is 81-foldlower than that of conventional Taq DNA Polymerase. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer/template). This kit contains twotypes of monoclonal antibodies that inhibit the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, enabling it toperform hot start PCR with great specificity. It contains all required reaction components (Phanta Flash Super-Fidelity DNA Polymerase,dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. This product contains tracking dyes, so PCR products can be directly loaded for electrophoresis after the reaction.Amplification will generate blunt-ended products, which are compatible with ClonExpress kits (Vazyme #C112/C113/C116) and TOPOcloning kit (Vazyme #C603).
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