VISTA Antibody / GI24

SKU: orb2642895

描述

VISTA / GI24 is a transmembrane protein expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESC's, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V-type Ig-like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30kDa extracellular fragment plus a 25-30kDa membrane-bound fragment. VISTA is a negative checkpoint regulator and is expressed on myeloid cells, T-cells and human TILs (tumor infiltrating lymphocytes) on MDSCs (myeloid-derived suppressor cells) in the TME (tumor microenvironment). It is very likely both a ligand and receptor and is a promising target for cancer immunotherapy.

图片与验证

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已测应用	ELISA, IHC-P
稀释范围	ELISA (order BSA/sodium azide-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
反应性	Human
应用说明	Optimal dilution of the VISTA antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG2b, kappa
克隆号	VISTA/3006
免疫原	A recombinant full-length human VISTA protein was used as the immunogen for the VISTA antibody.
纯化	Protein G affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
有效期	12 months from date of receipt.
免责声明	For research use only

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VISTA Antibody / GI24 [orb607155]
应用：
ELISA, IHC-P
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 μg, 20 μg
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VISTA Antibody / GI24 [orb2642898]
应用：
ELISA, IHC-P
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
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Unconjugated
可选规格： 100 μg
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VISTA Antibody / GI24 [orb607154]
应用：
ELISA, IHC-P
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 μg, 20 μg
Item 1 of 3
VISTA Antibody / GI24 [orb2642897]
应用：
IHC-P
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 7 ml
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VISTA Antibody / GI24 [orb639922]
应用：
ELISA
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 μg, 20 μg

质量保证

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