Biotin-11-dUTP

SKU: orb1733881

描述

Biotin-11-dUTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of uridine. Recommended Biotin-11-dUTP/dTTP ratio for PCR and Nick Translation: 50% Biotin-11-dUTP/50% dTTP.

图片与验证

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应用说明	Incorporation into DNA/cDNA by: PCR with Taqpolymerase & in-house data; Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow 3'-5' exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT); Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: λmax 240/289 nm, ε 10.7/7.1 L mmol-1 cm-1, (Tris-HCl pH 7.5).

关键属性

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MW	Theoretical MW: 862.67 g/mol (free acid); Detected MW: 862.18 g/mol (free acid)
纯度	≥ 95% (HPLC)
分子式	C₂₈H₄₅N₆O₁₇P₃S
SMILES	C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NC/C=C/c1c(=O)[nH]c(=O)n([C@H]3C[C@@H]([C@H](O3)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)O)c1)NC(=O)N2

储存与操作

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储存	store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Biotin-X-(5-aminoallyl)-dUTP, γ-[N-(Biotin-6-amino-hexanoyl)]-(5-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt

相似产品

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Item 1 of 1
Biotin-11-dUTP [orb64042]
纯度：
≥ 95% (HPLC)
MW:
Theoretical MW: 862.67 g/mol (free acid); Detected MW: 862.18 g/mol (free acid)
分子式：
C₂₈H₄₅N₆O₁₇P₃S
可选规格： 5 x 200 μl
Biotin-11-dUTP [orb1690207]
CAS 号：
86303-25-5
MW:
862.67
分子式：
C₂₈H₄₅N₆O₁₇P₃S
可选规格： 25 mg

质量保证

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化合物标识

PubChem CID

6436394 ↗

关键属性

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暂无计算属性。

实验方案信息

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纯度：	≥ 95% (HPLC)
MW:	Theoretical MW: 862.67 g/mol (free acid); Detected MW: 862.18 g/mol (free acid)
分子式：	C₂₈H₄₅N₆O₁₇P₃S

CAS 号：	86303-25-5
MW:	862.67
分子式：	C₂₈H₄₅N₆O₁₇P₃S

Biotin-11-dUTP

描述

图片与验证

关键属性

储存与操作

别名

相似产品

Biotin-11-dUTP [orb64042]

Biotin-11-dUTP [orb1690207]

质量保证

化合物标识

关键属性

实验方案信息

可选规格