Biotin-11-dUTP

SKU: orb64042

描述

Biotin-11-dUTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of uridine. Recommended Biotin-11-dUTP/dTTP ratio for PCR and Nick Translation: 50% Biotin-11-dUTP/50% dTTP.

图片与验证

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Item 1 of 1

应用说明	Incorporation into DNA/cDNA by: PCR with Taqpolymerase & in-house data; Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow 3'-5' exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT); Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: λmax 240/289 nm, ε 10.7/7.1 L mmol-1 cm-1, (Tris-HCl pH 7.5).

关键属性

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MW	Theoretical MW: 862.67 g/mol (free acid); Detected MW: 862.18 g/mol (free acid)
纯度	≥ 95% (HPLC)
分子式	C₂₈H₄₅N₆O₁₇P₃S
SMILES	C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NC/C=C/c1c(=O)[nH]c(=O)n([C@H]3C[C@@H]([C@H](O3)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)O)c1)NC(=O)N2

客户验证数据

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以下数据由各地研究人员提交。即使我们尚未自行检测，这些结果也有助于判断该产品是否适合您的应用或种属。

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反应性分布

最新实验

5 Results

稀释

样本

摘要

DNA end biotinylation

Bacteriophage

Purified linear DNA constructs

Biotin-11-dUTP is used for enzymatic end-labeling...

PCR amplicon biotinylation

Bacteria

2–20 μM

Purified genomic DNA

Biotin-11-dUTP is used for PCR-based DNA...

Probe labeling for hybridization

Human

Genomic DNA isolated from peripheral blood leukocytes of cystic fibrosis patients and healthy controls.

Biotin-11-dUTP is used for nick translation-based...

DNA end labeling (TdT-based)

Mammalian

Fixed cellular specimens containing genomic DNA

Biotin-11-dUTP is used for probe labeling...

RNA / cDNA labeling

Synthetic RNA pool

20 μM

Hydrolyzed RNA pools and selected functional RNA fragments, which are processed into cDNA after selection.

Biotin-11-dUTP is used during reverse transcription–based...

储存与操作

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储存	store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Biotin-X-(5-aminoallyl)-dUTP, γ-[N-(Biotin-6-amino-hexanoyl)]-(5-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt

相似产品

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Biotin-11-dUTP [orb1733881]
纯度：
≥ 95% (HPLC)
MW:
Theoretical MW: 862.67 g/mol (free acid); Detected MW: 862.18 g/mol (free acid)
分子式：
C₂₈H₄₅N₆O₁₇P₃S
可选规格： 200 μl
Biotin-11-dUTP [orb1690207]
CAS 号：
86303-25-5
MW:
862.67
分子式：
C₂₈H₄₅N₆O₁₇P₃S
可选规格： 25 mg

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

化合物标识

PubChem CID

6436394 ↗

关键属性

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暂无计算属性。

实验方案信息

查看更多实验方案

纯度：	≥ 95% (HPLC)
MW:	Theoretical MW: 862.67 g/mol (free acid); Detected MW: 862.18 g/mol (free acid)
分子式：	C₂₈H₄₅N₆O₁₇P₃S

CAS 号：	86303-25-5
MW:	862.67
分子式：	C₂₈H₄₅N₆O₁₇P₃S

Biotin-11-dUTP

描述

图片与验证

关键属性

客户验证数据

按应用统计成功数

反应性分布

最新实验

储存与操作

别名

相似产品

Biotin-11-dUTP [orb1733881]

Biotin-11-dUTP [orb1690207]

质量保证

化合物标识

关键属性

实验方案信息

可选规格