Biotin-14-dATP

SKU: orb1733817

描述

Biotin-14-dATP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties for Nick Translation are ensured by a 14-atom linker attached to the N6 position of adenine. For PCR incorporation experiments e.g. with Taqpolymerase Biotin-11-dATP is recommended whose Biotin moiety is attached to the N7-Deaza position of adenine via a 11-atom linker. Recommended Biotin-14-dATP/dATP ratio for Nick Translation: 50% Biotin-14-dATP/50% dATP.

图片与验证

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应用说明	Incorporation into DNA/cDNA by: Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow fragment. Spectroscopic Propertie: λmax 266 nm, ε 16.2 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

关键属性

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MW	Theoretical MW: 929.81 g/mol (free acid); Detected MW: 929.27 g/mol (free acid)
纯度	≥ 95% (HPLC)
分子式	C₃₂H₅₄N₉O₁₅P₃S
SMILES	OP(=O)(O)OP(=O)(O)OP(=O)(O)OC[C@H]1O[C@@H](n2cnc3c(NCCCCCCNC(=O)CCCCCNC(=O)CCCC[C@@H]4SC[C@@H]5NC(=O)N[C@H]45)ncnc23)C[C@@H]1O

储存与操作

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储存	store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Biotin-14-N6-(6-Aminohexyl)-dATP, Triethylammonium salt, Bio-14-dATP

相似产品

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Item 1 of 1
Biotin-14-dATP [orb533181]
纯度：
≥ 95% (HPLC)
MW:
Theoretical MW: 929.81 g/mol (free acid); Detected MW: 929.27 g/mol (free acid)
分子式：
C₃₂H₅₄N₉O₁₅P₃S
可选规格： 5 x 200 μl

质量保证

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化合物标识

PubChem CID

176417988 ↗

关键属性

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暂无计算属性。

实验方案信息

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纯度：	≥ 95% (HPLC)
MW:	Theoretical MW: 929.81 g/mol (free acid); Detected MW: 929.27 g/mol (free acid)
分子式：	C₃₂H₅₄N₉O₁₅P₃S

Biotin-14-dATP

描述

图片与验证

关键属性

储存与操作

别名

相似产品

Biotin-14-dATP [orb533181]

质量保证

化合物标识

关键属性

实验方案信息

可选规格