Biotin-14-dATP

SKU: orb533181

描述

Biotin-14-dATP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties for Nick Translation are ensured by a 14-atom linker attached to the N6 position of adenine. For PCR incorporation experiments e.g. with Taqpolymerase Biotin-11-dATP is recommended whose Biotin moiety is attached to the N7-Deaza position of adenine via a 11-atom linker. Recommended Biotin-14-dATP/dATP ratio for Nick Translation: 50% Biotin-14-dATP/50% dATP.

图片与验证

−

Item 1 of 1

应用说明	Incorporation into DNA/cDNA by: Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow fragment. Spectroscopic Propertie: λmax 266 nm, ε 16.2 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

关键属性

−

MW	Theoretical MW: 929.81 g/mol (free acid); Detected MW: 929.27 g/mol (free acid)
纯度	≥ 95% (HPLC)
分子式	C₃₂H₅₄N₉O₁₅P₃S
SMILES	OP(=O)(O)OP(=O)(O)OP(=O)(O)OC[C@H]1O[C@@H](n2cnc3c(NCCCCCCNC(=O)CCCCCNC(=O)CCCC[C@@H]4SC[C@@H]5NC(=O)N[C@H]45)ncnc23)C[C@@H]1O

客户验证数据

−

以下数据由各地研究人员提交。即使我们尚未自行检测，这些结果也有助于判断该产品是否适合您的应用或种属。

按应用统计成功数

反应性分布

最新实验

6 Results

稀释

样本

摘要

Labelling

Mouse

G1E-ER4 erythroblast cell line and its derived sublines

It serves as a biotinylated nucleotide...

HiChIP

Mouse

3134 mouse mammary carcinoma cell line

It is used during a 1-hour...

Chemical Reagent

Human

A549-ACE2 cells

It is used as a chemical...

DNA 3′- end labeling

Schmidtea mediterranea

Worm lysates

Biotin-14-dATP was used by terminal transferase...

Hybridization-based RNA capture

Archaea

Synthetic antisense oligonucleotides, Cellular RNA transcripts

Biotin-14-dATP is incorporated at the 3′...

储存与操作

−

储存	store at -20 °C
有效期	12 months from date of receipt.
危害信息	Non-Toxic
免责声明	For research use only

别名

−

Biotin-14-N6-(6-Aminohexyl)-dATP, Triethylammonium salt, Bio-14-dATP

相似产品

−

Biotin-14-dATP [orb1733817]
纯度：
≥ 95% (HPLC)
MW:
Theoretical MW: 929.81 g/mol (free acid); Detected MW: 929.27 g/mol (free acid)
分子式：
C₃₂H₅₄N₉O₁₅P₃S
可选规格： 200 μl

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

化合物标识

PubChem CID

176417988 ↗

关键属性

−

暂无计算属性。

实验方案信息

查看更多实验方案

纯度：	≥ 95% (HPLC)
MW:	Theoretical MW: 929.81 g/mol (free acid); Detected MW: 929.27 g/mol (free acid)
分子式：	C₃₂H₅₄N₉O₁₅P₃S

Biotin-14-dATP

描述

图片与验证

关键属性

客户验证数据

按应用统计成功数

反应性分布

最新实验

储存与操作

别名

相似产品

Biotin-14-dATP [orb1733817]

质量保证

化合物标识

关键属性

实验方案信息

可选规格