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Cytokeratin 18 Antibody Cocktail

SKU: orb2638782

描述

This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

图片与验证

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已测应用IHC-P
稀释范围The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
反应性Human
应用说明
Optimal dilution of the Cytokeratin 18 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型Primary Antibody
宿主Mouse
克隆性Monoclonal
同种型Mouse IgG1, kappa
克隆号Clone Cocktail
免疫原Recombinant full-length human KRT18 protein was used as the immunogen for the Cytokeratin 18 antibody cocktail.
纯化Protein G affinity chromatography
偶联Unconjugated

储存与操作

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储存Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
有效期12 months from date of receipt.
免责声明For research use only

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质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

UniProt 详情

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暂无 UniProt 数据

实验方案信息

IHC-P
石蜡切片免疫组化
查看实验方案

可选规格

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7 ml
¥ 9,750.00
免费二抗(20 µl)0/0

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发货Usually dispatched within 5-10 working days
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