Cytokeratin 8/18 Antibody Cocktail

SKU: orb749951

描述

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained. Immuno-histochemical staining with this mAb is indistinguishable from that obtained with monoclonal antibody 5D3.

研究领域

Epigenetics, Infectious Diseases

图片与验证

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已测应用	IHC-P
稀释范围	Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
反应性	Human
应用说明	Optimal dilution of the Cytokeratin 8/18 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG1, kappa
克隆号	KRT8/803 + KRT18/835
免疫原	Recombinant full-length human KRT8 protein (KRT8/803) and KRT18 protein (KRT18/835) were used as the immunogen for the Cytokeratin 8/18 antibody cocktail.
纯化	Protein G affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
有效期	12 months from date of receipt.
免责声明	For research use only

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Cytokeratin 8 Antibody Cocktail [orb248440]
应用：
FACS, IF, IHC-P, WB
反应性：
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种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 μg, 20 μg
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Cytokeratin 8 Antibody Cocktail [orb2637813]
应用：
FACS, IF, IHC-P, WB
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种属/宿主：
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可选规格： 100 μg
Item 1 of 5
Cytokeratin 8/18 Antibody Cocktail [orb2639610]
应用：
IHC-P
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 7 ml
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Cytokeratin 8/18 Antibody Cocktail [orb2639611]
应用：
IHC-P
反应性：
Human
种属/宿主：
Mouse
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Monoclonal
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Unconjugated
可选规格： 100 μg
Item 1 of 4
Cytokeratin 8/18 Antibody Cocktail [orb248628]
应用：
FACS, IF, IHC-P, WB
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 μg, 20 μg

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

UniProt

P05783, P05787

UniProt 详情

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暂无 UniProt 数据

实验方案信息

IHC-P

石蜡切片免疫组化

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可选规格

请在下方选择规格

20 μg

¥ 4,810.00

100 μg

¥ 9,750.00

选择偶联或无载体版本

免费二抗（20 µl）0/0

请先将抗体产品加入购物车。

数量

Usually dispatched within 5-10 working days

批量询价

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应用：	FACS, IF, IHC-P, WB
反应性：	Human, Rat
种属/宿主：	Mouse
克隆性：	Monoclonal
偶联：	Unconjugated