Cytokeratin 8/18 Antibody Cocktail

SKU: orb248628

描述

Cytokeratins are a group of proteins expressed in pairs in keratinized and non-keratinized epithelial tissue. Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). The pair are normally seen in simple but not stratified epithelial cells. This antibody cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. Immunohistochemical staining with this antibody cocktail is indistinguishable from that obtained with monoclonal antibody 5D3.

图片与验证

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已测应用	FACS, IF, IHC-P, WB
稀释范围	Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
反应性	Human
应用说明	Differences in protocols and secondaries may require the Cytokeratin 8/18 antibody to be titered for optimal performance.1. FFPE staining requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

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抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG1, kappa
克隆号	K8.8 + DC10
免疫原	A keratin preparation from a human carcinoma (K8.8) and PMC-42 human breast carcinoma cells (DC10) were used as the immunogen for this Cytokeratin 8/18 antibody cocktail.
纯化	Protein G affinity chromatography
偶联	Unconjugated

储存与操作

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储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
有效期	12 months from date of receipt.
免责声明	For research use only

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质量保证

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