DYKDDDDK Tag (FLAG) Antibody

SKU: orb345396

该产品可以定制

KO/KD
已验证

描述

Detection of FLAG proteins antibody

图片与验证

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Item 1 of 9

已测应用	ELISA, KO/KD Validated, WB
稀释范围	ELISA: 1:20,000 - 1:40,000, WB: 1:2,000 - 1:10,000
应用说明	This antibody is optimally suited for monitoring the expression of FLAG tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and FLAGä containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK. Biorbyt's polyclonal antibody to detect FLAG conjugated proteins binds FLAG containing fusion proteins with greater affinity than the widely used monoclonal M1, M2 and M5 clones, and shows greater sensitivity in most assays. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.

关键属性

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抗体类型	Primary Antibody
宿主	Rabbit
克隆性	Polyclonal
同种型	IgG
免疫原	This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. This antibody reacts with FLAG conjugated proteins.
纯度	This affinity purified antibody is directed against the FLAG motif and is useful in determining its presence in various assays. This polyclonal anti-FLAG tag antibody detects over-expressed proteins containing the FLAG epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
偶联	Unconjugated

储存与操作

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储存	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
形态/外观	Liquid (sterile filtered)
缓冲液/防腐剂	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度	1.09
有效期	12 months from date of receipt.
干冰运输	请注意：本产品需要干冰运输，将收取干冰附加费。
免责声明	For research use only

别名

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rabbit antibody for the detection of FLAG conjugated proteins, rabbit anti DYKDDDDK

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