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KO/KD
已验证
已验证
描述
图片与验证
−
Item 1 of 9
| 已测应用 | ELISA, KO/KD Validated, WB |
|---|---|
| 稀释范围 | ELISA: 1:90,000 - 1:250,000, WB: 1:2,000 - 1:10,000 |
| 应用说明 |
关键属性
−| 抗体类型 | Primary Antibody |
|---|---|
| 宿主 | Rabbit |
| 克隆性 | Polyclonal |
| 同种型 | IgG |
| 免疫原 | This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. This antibody reacts with FLAG conjugated proteins. |
| 纯度 | This affinity purified antibody is directed against the FLAG motif and is useful in determining its presence in various assays. This polyclonal anti-FLAG tag antibody detects over-expressed proteins containing the FLAG epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. |
| 偶联 | Unconjugated |
储存与操作
−| 储存 | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| 形态/外观 | Liquid (sterile filtered) |
| 缓冲液/防腐剂 | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| 浓度 | 1.09 |
| 有效期 | 12 months from date of receipt. |
| 干冰运输 | 请注意：本产品需要干冰运输，将收取干冰附加费。 |
| 免责声明 | For research use only |
别名
−rabbit antibody for the detection of FLAG conjugated proteins, rabbit anti DYKDDDDK
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![Anti-DDDDK-tag [M2.1]](/images/pub/media/catalog/product/NewWebsite/35/orb396135_1.png)
![Anti-DDDDK-tag [M2.1]](/images/pub/media/catalog/product/NewWebsite/35/orb396135_2.png)
![Anti-DDDDK-tag [M2.1]](/images/pub/media/catalog/product/NewWebsite/35/orb396143_1.png)
![Anti-DDDDK-tag [M2.1]](/images/pub/media/catalog/product/NewWebsite/35/orb396143_2.png)
质量保证
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.