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F(ab')2 Mouse IgG (H&L) Antibody Pre-Adsorbed

SKU: orb348225

描述

F(ab')2 MOUSE IgG (H&L) antibody

图片与验证

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已测应用ELISA, IHC, WB
稀释范围ELISA: 1:20,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
反应性Mouse
应用说明
F(ab')2 Anti-Mouse IgG Antibody has been tested by ELISA and SDS-PAGE and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

关键属性

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抗体类型Secondary Antibody
宿主Goat
克隆性Polyclonal
同种型IgG F(ab')2
免疫原Mouse IgG whole molecule
纯度This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Human Serum Proteins.
偶联Unconjugated

储存与操作

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储存Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
形态/外观Liquid (sterile filtered)
缓冲液/防腐剂Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度1.0 mg/mL
有效期12 months from date of receipt.
免责声明For research use only

别名

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Goat F(ab')2 Anti-Mouse IgG Antibody Pre-Adsorbed, Goat Fab2 Anti-Mouse IgG Antibody

相似产品

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质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

实验方案信息

WB
免疫印迹(WB)
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IHC
免疫组化
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ELISA
酶联免疫吸附测定(ELISA)
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可选规格

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1 mg
¥ 5,070.00
免费二抗(20 µl)0/0

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发货Usually dispatched within 5-10 working days
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