F(ab')2 Rabbit IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

SKU: orb348316

该产品可以定制

描述

F(ab')2 Rabbit IgG (H&L) antibody (RPE)

图片与验证

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已测应用	FC, IF, WB
稀释范围	FC: 1:100 - 1:250, IF: 1:100 - 1:250
反应性	Rabbit
应用说明	F(ab')2 Anti-Rabbit IgG Phycoerythrin Antibody has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

关键属性

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抗体类型	Secondary Antibody
宿主	Goat
克隆性	Polyclonal
同种型	IgG F(ab')2
免疫原	Rabbit IgG whole molecule
纯度	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
偶联	RPE

储存与操作

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储存	Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
形态/外观	Lyophilized
缓冲液/防腐剂	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度	0.5 mg/mL
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Goat F(ab')2 Anti-Rabbit IgG Phycoerythrin Conjugated Pre-Adsorbed Antibody, Goat Fab2 Anti-Rabbit IgG Antibody PE Conjugation

相似产品

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F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed [orb348280]
应用：
DOT, FC, IF
反应性：
Mouse
种属/宿主：
Rabbit
克隆性：
Polyclonal
偶联：
RPE
可选规格： 500 μg
Item 1 of 1
F(ab')2 Rabbit IgG (H&L) Antibody Phycoerythrin Conjugated Pre-adsorbed [orb348326]
应用：
FC, IF, WB
反应性：
Rabbit
种属/宿主：
Donkey
克隆性：
Polyclonal
偶联：
RPE
可选规格： 500 μg
F(ab')2 Rat IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed [orb348368]
反应性：
Rat
种属/宿主：
Rabbit
克隆性：
Polyclonal
偶联：
RPE
可选规格： 500 μg

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

实验方案信息

免疫印迹（WB）

流式细胞术

免疫荧光

应用：	DOT, FC, IF
反应性：	Mouse
种属/宿主：	Rabbit
克隆性：	Polyclonal
偶联：	RPE

F(ab')2 Rabbit IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed

描述

图片与验证

关键属性

储存与操作

别名

相似产品

F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed [orb348280]

F(ab')2 Rabbit IgG (H&L) Antibody Phycoerythrin Conjugated Pre-adsorbed [orb348326]

F(ab')2 Rat IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed [orb348368]

质量保证

实验方案信息

可选规格