GDC-0575

SKU: orb1299941

描述

GDC-0575

研究领域

Cell Biology

图片与验证

−

关键属性

−

CAS 号	1196541-47-5
MW	378.27
纯度	98.00% (May vary between batches)
分子式	C₁₆H₂₀BrN₅O
SMILES	N[C@@H]1CCCN(C1)c1c(Br)cnc2[nH]cc(NC(=O)C3CC3)c12
靶点	Chk
溶解性	10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.29 mM);DMSO:50 mg/mL (132.18 mM)

生物活性

−

靶点 IC50	Chk1:1.2 nM
体内	CHK1 inhibitor (CHK1i) GDC-0575 enhances AraC-mediated killing of AML cells both in vitro and in vivo, thus abrogating any potential chemoresistance mechanisms involving DNA repair. Importantly, this combination of drugs does not affect normal long-term hematopoietic stem/progenitors.
体外	GDC-0575 demonstrates markedly higher efficacy in inducing DNA damage, replication stress, and cell death compared to V158411, LY2603618, and MK-8776 in a panel of melanoma cell lines.
动物研究	For in vivo experiments, aliquots of GDC-0575 (10 mg/mL) were stored at 20℃and diluted in 100 mM sodium citrate buffer immediately prior to each experiment. GDC-0575 was used at 1.8 mg/ml for female mice (~25 g) and 2.6 mg/ml for male mice (~35 g) via oral gavage (final concentration 7.5 mg/kg). For in vitro experiments, aliquots of GDC-0575 (100 μM) were stored at 20 ℃ and used at a final concentration of 100 nM. AraC (Cytosine β-D-arabinofuranoside C1768, Sigma) was used at 10 mg/kg (commonly used in clinical practice) for in vivo and at 100 nM and 500 nM for in vitro experiments. For in vivo experiments, toxicity of GDC-0575 was assessed in non-engrafted NSG mice using a range of concentrations of GDC-0575 in combination with AraC. 7.5 mg/kg GDC-0575 was the highest concentration to have no significant or lasting adverse effects in mice. Similarly, for in vitro experiments, 100 nM GDC-0575 in combination with 500 nM AraC was the highest concentration non-cytotoxic to MS5 stoma cell. ATR inhibitor was used at a final concentration of 0.5 μM.

储存与操作

−

储存	-20°C
有效期	12 months from date of receipt.
免责声明	For research use only

别名

−

ARRY 575, ARRY575, ARRY-575, Chk1, Checkpoint Kinase (Chk), inhibit, Inhibitor, GDC0575, GDC-0575, GDC 0575, RG 7741, RG7741, RG-7741

相似产品

−

GDC0575 monohydrochloride [orb1305087]
纯度：
97.85% (May vary between batches)
CAS 号：
1196504-54-7
MW:
414.73
分子式：
C₁₆H₂₁BrClN₅O
可选规格： 100 mg, 50 mg, 2 mg, 5 mg, 10 mg, 25 mg, 1 ml x 10 mM (in DMSO), 200 mg, 1 mg
GDC-0575 dihydrochloride [orb1687308]
CAS 号：
1657014-42-0
MW:
451.19
分子式：
C₁₆H₂₂BrCl₂N₅O
可选规格： 2 mg
GDC-0575 [orb1226825]
纯度：
>98% (HPLC)
CAS 号：
1196541-47-5
MW:
377.286
分子式：
C₁₇H₂₁BrN₄O
可选规格： 10 mg, 25 mg, 5 mg, 50 mg, 100 mg, 200 mg, 500 mg, 1 g

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

化合物标识

PubChem CID

46917793 ↗

关键属性

−

暂无计算属性。

实验方案信息

查看更多实验方案

纯度：	97.85% (May vary between batches)
CAS 号：	1196504-54-7
MW:	414.73
分子式：	C₁₆H₂₁BrClN₅O

CAS 号：	1657014-42-0
MW:	451.19
分子式：	C₁₆H₂₂BrCl₂N₅O

纯度：	>98% (HPLC)
CAS 号：	1196541-47-5
MW:	377.286
分子式：	C₁₇H₂₁BrN₄O

GDC-0575

描述

研究领域

图片与验证

关键属性

生物活性

储存与操作

别名

相似产品

GDC0575 monohydrochloride [orb1305087]

GDC-0575 dihydrochloride [orb1687308]

GDC-0575 [orb1226825]

质量保证

化合物标识

关键属性

实验方案信息

可选规格